Oxford Nanopore Technologies plc MNT-001 MinIT User Manual

Oxford Nanopore Technologies Limited MinIT Users Manual

Users Manual

D-0184-1, Rev 1.Quick start installation guide
WelcomeThe MinIT is made by: Oxford Nanopore Technologies Gosling Building, Edmund Halley Road Oxford Science Park OX4 4DQ, United Kingdom The product is protected by the patents and patents pending https://nanoporetech.com/patents Pre-installationBefore you install the device the following should be available: MinITPower SupplyWireless-Compatible Tablet, Laptop or SmartPhone1 x Oxford Nanopore MinION™MinION USB 3.0 CableOptional: USB Memory Stick (for Data Transfer)Optional: 1 x 1 GB Ethernet (for Networking)Here’s everything you need to install your device and to check the system is ready for use. Just follow the steps on the following pages in order to:• Setup the MinIT™ for use• Power on the device• RuntheHardwarechecktoconrmthe device has shipped successfully
Technical Specification: Recommendations: Place the MinIT on a stable and clean laboratory bench. Ensure the Ethernet and power connectors are in close proximity to where the MinIT will be installed.Sequencing should be carried out at a room temperature at +18° C to +25° C.Do not cover the ventilation grills.About your MinIT Usage adviceComponent SpecificationSize W 65 x L 120 X D 40 mmWeight 290 gCompute SpecicationProcessing: GPU accelerators (ARM Processor 6 Cores, 256 Core GPU), 8 GB RAMConnections 1 x USB 3.0 1 x USB 2.01 x 1 GB Ethernet (for Networking)On-Board SoftwareLinux OS and MinIT OS (including MinKNOW)
Your MinITWhat’s in the box: 1 x MinIT Device1 x Power supply4xCountry-SpecicAdapters
Useful information about MinIT setup:• GUIisdenedasaGraphicalUserInterface • For Windows OS, please refer to A4 to A6 later in this document• For Mac OS, please refer to B4 to B6 later in this document White LED display indicates the device is powered BlueLEDdisplay‘ash’indicatesthe Wi-Fi is being enabled GreenLEDdisplay‘ash’indicatestheWi-Fi has connected successfully RedLEDdisplay‘ash’indicatesthe Wi-Fi connection has not been successful.MinIT overviewIMPORTANT:Any further information or functional changes to the Quick Start Installation Guide, can be found on the MinIT channel in the Oxford Nanopore Technologies Community, here:https://community.nanoporetech.com/posts/channels/minit
Setting up your MinIT 1 Unpack the device Place the MinIT on a clean surface.2 Attach the required cables 1 x MinION via USB 2.0 port (lower of two, power port right) 1 x (Optional) USB SSD Storage 1 x (Optional) Ethernet 3 Power Supply Connect the power supply to the AC mains power and the MinIT. The MinIT logo will illuminate white when powered.• Ethernet• USB 2.0 (for MinION)• USB 3.0 (for SSD storage)• Power socket
Connecting your MinIT – Windows OSA4 Identify the MinIT ID The ID for the MinIT is shown above in the red box, on the underside label. Note this number (e.g. MT-110087) is required for Wi-Fi connection.A5 Connect to the MinIT (Wi-Fi) Using a Wi-Fi compatible device, connect to the Wi-Fi with the ID of your MinIT (A4). Login with the Security Key: WarmButterflyWings98A6 Opening the MinKNOW GUI Open network sharing and go to \\mt-1XXXXX(MinITID(A4)).ThisisyourMinIT’slocation on the network. In the Data folder, open the MinKNOW™ shortcut to open the MinKNOW GUI.
Connecting your MinIT – Mac OSB4 Identify the MinIT ID The ID for the MinIT is shown adjacent in the red box, on the underside label. Note this number (e.g. MT-110087) is required for Wi-Fi connection.B5 Connect to the MinIT (Wi-Fi) Using a Wi-Fi compatible device, connect to the Wi-Fi with the ID of your MinIT (B4). Login with the Password: WarmButterflyWings98B6 Opening the MinKNOW GUI Under Shared, click on MT-1XXXXX (MinITID(B4)).ThisisyourMinIT’slocation on the network. In the Data folder, open the MinKNOW shortcut to open the MinKNOW GUI.
Running the Hardware Check7 Plug MinION Into MinIT Plug the MinION into the USB 2.0 port andinsertyourCTCowcellintotheMinION. 8 Select CTC The CTC will appear numerically in the GUI.Selectthe‘owcell’iconbytickingthe box below it.9 Click the “Check hardware” button This can be found at the bottom of the screen, shown in the red box above.USB 2.0
10 Confirm the CTC is loaded correctly A display will appear asking you to check the CTC is inserted correctly. Once conrmedclick“Starttest”.11 Check the messages The message tab on the right will show “Calibrationnishedsuccessfully”,followedby“Hardwarecheckhascompletedsuccessfully”.12 Your hardware is working correctly Oncethecongurationrunhascompleted, the Hardware is ready for a sequencing experiment. Please refer to the Nanopore Community for additional protocols.
Powering off the device13 Close the GUI Ensure the MinION is not running. Close theGUIthroughthe“x”inthetoprightcorner.14 Power off device Remove MinION from MinIT and switch power off at the power source. The power cable can then be removed from the MinIT.
Oxford Nanopore Technologies, the Wheel icon, MinION, MinKNOW and MinIT are registered trademarks of Oxford Nanopore Technologies in various countries. © Copyright 2018 Oxford Nanopore Technologies. MinIT is currently for research use only. D-0184-1, Rev 1.Additional information Safety InformationThe MinIT is for Research Use Only.Please ensure you have reviewed the MinIT safety information before you start using the device.For more the fully updated Start Guide technical information, protocols, safety guides see:https://community.nanoporetech.com/posts/channels/minitCleaning the MinITShould any leaks develop over the course of loading aowcell,orduringanexperiment, please absorb excess liquid using a laboratory tissue and clean the area using an IPA wipe. Please dispose of the cleaning materials into a biohazard waste bin.

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