Leica Microsystems EZ4W Stereo Microscope with integrated Camera User Manual 15 EZ4 W UserMan

Leica Microsystems (Schweiz) AG Stereo Microscope with integrated Camera 15 EZ4 W UserMan

Contents

15_EZ4 W UserMan

Leica ES2Leica EZ4Leica EZ4 WUser Manual
Leica E Series User Manual 2Congratulations!You have just purchased a Leica E Series stereo-microscope – an excellent choice! Viewing entire objects under magnification imparts perspectives and discoveries that would not be possible with the naked eye. In developing our stereomicroscopes, we have placed great emphasis on simple, self-explan-atory operation. However, please take the time to read the instruction manual and the notes on operating safety, to learn about all the features and capabilities of your instrument so that you can use it safely and to its best advantage. Should you have any questions, please consult your local Leica representative. We are gladly at your service!
Leica E Series User Manual 3Chapter OverviewSafety and Use 6Leica ES2 13Leica EZ4 and Leica EZ4 W 21Eyepieces (only for Leica EZ4) 32Photography Using the Leica EZ4 W 40Get Set! 46The Camera Remote Control (Optional) 54Care, Transport, Contact Persons 67Specifications 69Dimensions 71
Leica E Series User Manual 4Safety and Use Symbols Used 7Safety Instructions 8FCC Requirements for Operation in the United States 10Instructions for Use 11Leica ES2 Overview: Leica ES2 14Transport, Placement and Storage 15The Correct Interpupillary Distance 16Using the LED Illuminator 17Focusing 18Magnification Display 19Adjusting the Resistance of the Focus Drive 20Leica EZ4 and Leica EZ4 W Overview: Leica EZ4 22Instrument Variant: Leica EZ4 W 23Transport, Placement and Storage 24The Correct Interpupillary Distance 25Switching on the LED Illuminator 26Controlling the Automatic Shutoff 27Illumination Types 28Focusing 29ContentsMagnification Display 30Adjusting the Resistance of the Focus Drive 31Eyepieces (Only for Leica EZ4) Changing Eyepieces (Only for Leica EZ4, Open Version) 33Eyepieces and Eyecups (Only for Leica EZ4, Open Version) 34Dioptric Correction (Only for Leica EZ4, Open Version) 35Graticules (Only for Leica EZ4, Open Version) 37Graticules: Preparation 38Inserting the Graticules 39Photography Using the Leica EZ4 W Introduction 41The Basics 42Leica EZ4 W: Overview 43Unpacking 45Get Set! USB Mode – Capturing and Adjusting Images Using a Computer 47SD Mode – Capturing Images Without a Computer or Without a Wireless Device 49Adjusting Settings While in SD Mode 51WiFi Mode – Capturing and Adjusting Images Using a Wireless Device 52Ethernet Mode – Capturing and Adjusting Images Using a Network 53
Leica E Series User Manual 5Contents (Continued)The Camera Remote Control (Optional) Optional Remote Control 55Viewing Images and Movies With the Optional Remote Control 56Calling up the Camera Menu 57COLOR (Automatic White Balance) 58COLOR (Manual White Balance) 59EXPOSURE 60RESOLUTION 61SETUP CAMERA (Camera Settings) 62SETUP ETHERNET 63SETUP WiFi 64Pairing Cameras With Remotes 66Care, Transport, Contact Persons Care, Transport, Contact Persons 68Specifications Camera Specifications 70Dimensions Leica ES2 72Leica EZ4 W 74Leica EZ4, 10× Eyepieces 76Leica EZ4 without Eyepieces 78
Leica E Series User Manual Safety and Use 6Safety and Use
Leica E Series User Manual Safety and Use 7Symbols Used Read and understand user manual before using this device.Warning of a danger•This symbol indicates especially impor-tant information that must be read and complied with. Failure to comply can cause the following: OHazards to personnel OFunctional disturbances or damaged inst-rumentsWarning of hazardous electrical voltageThis symbol indicates especially impor-tant information that, if not observed, can cause the following: OHazards to personnel OFunctional disturbances or damaged inst-rumentsDanger due to hot surface This symbol warns against touching hot surfaces, e.g. those of light bulbs. Important information This symbol indicates additional infor-mation or explanations that intend to provide clarity.
Leica E Series User Manual Safety and Use 8Before installing, operating or using the instrument, it is mandatory to read this user manual. In particular, please observe all safety instructions.The "Safety Concept" brochure contains addi-tional safety information regarding service work, requirements and the handling of the microscope, accessories as well as general safety instructions.You can combine individual system articles with articles from external suppliers. Please read the user manual and the safety requirements of the supplier.To maintain the unit in its original condition and to ensure safe operation, the user must follow the instructions and warnings contained in these user manuals.Warning of a dangerThis symbol indicates especially impor-tant information that is mandatory to read and observe. Failure to follow these instructions may pose hazards to personnel, cause instrument malfunctions or damage the instrument.Hazardous electrical voltageThis symbol indicates especially impor-tant information that is mandatory to read and observe. Failure to follow these instructions may pose hazards to personnel, cause instrument malfunctions or damage the instrument.Important informationThis symbol indicates additional infor-mation or explanations that intend to provide clarity.EC Declaration of ConformityElectrically operated accessories are constructed based on the state of the art of technology and are provided with an EC Decla-ration of Conformity.Technical SafetyTo connect the Leica E Series microscopes to power, use a power cable that meets the power cable specifications in your country. The power socket used has to be equipped with a 16 A or 10 A fuse. Only connect the instrument to a grounded power socket that has been installed according to regulations. The power supply voltage has to correspond to the voltage speci-fied on the identification label. Make sure that the ground connection is neither defective nor interrupted, since otherwise a malfunction poses a risk of fatal injury. Never unplug the power plug with moist or wet hands. There is danger of an electric shock.Safety Instructions
Leica E Series User Manual Safety and Use 9Safety Instructions (Continued)Instrument safety and EMCOur device has been designed, produced and tested in compliance with OIEC EN 61010-1: Electrical equipment for measurement, control and laboratory use – Safety – Part one: general requirements OEN 60950-1: Information technology equip-ment – Safety – Part one: general require-ments ORadio interference suppression in compli-ance with EN 55011 class B ONoise immunity in compliance with DIN EN 61326-1.The device meets the requirements of EU directives O2006/95/EG Low voltage directive O2014/30/EU EMC directive O2011/65/EU RoHS directive and carries the CE mark. The device must be disposed of in accordance with 2012/19/EU WEEE directive.Intended for indoor use only in all EU member states, EFTA states and Switzerland.Leica EZ4 W OEN 300328: Electromagnetic compatibility and Radio spectrum Matters (ERM); Wide-band transmission systems; Data transmis-sion equipment operating in the 2,4 GHz ISM band and using wide band modulation techniques; Harmonized EN covering essen-tial requirements under article 3.2 of the R&TTE directive.
Leica E Series User Manual Safety and Use 10FCC Requirements for Operation in the United StatesFCC radio frequency interference warnings and instructionsThis equipment has been tested and found to comply with the limits for a Class B digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reason-able protection against harmful interference in a residential installation. This equipment generates, uses and can radiate radio frequency energy and, if not installed and used in accor-dance with the instructions, may cause harm-ful interference to radio communications. However, there is no guarantee that interfer-ence will not occur in a particular installation. If this equipment does cause harmful interfer-ence to radio or television reception, which can be determined by turning the equipment off and on, the user is encouraged to try to correct the interference by one or more of the follow-ing measures: OReorient or relocate the receiving antenna. OIncrease the separation between the equip-ment and receiver. OConnect the equipment into an outlet on a circuit different from that to which the receiver is connected. OConsult the dealer or an experienced radio/TV technician for help.FCC information to userThis product does not contain any user service-able components and is to be used with approved antennas only. Any product changes or modifications will invalidate all applicable regulatory certifica-tions and approvals.FCC guidelines for human exposureThis equipment complies with FCC radiation exposure limits set forth for an uncontrolled environment. This equipment should be installed and operated with minimum distance of 20 cm between the radiator and your body.This transmitter must not be co-located or oper-ating in conjunction with any other antenna or transmitter. FCC caution OAny changes or modifications not expressly approved by the party responsible for compliance could void the user's authority to operate this equipment. OThis device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) This device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation. OThis device and its antenna(s) must not be co-located or operation in conjunction with any other antenna or transmitter.
Leica E Series User Manual Safety and Use 11Intended useThe E Series microscopes are optical instru-ments for improving the visibility of objects through magnification and illumination. It is used for observing and documenting.Place of useUse the instrument in enclosed, dust-free rooms at +10°C to +40 °C only. Protect it from oil, chemicals and extreme humidity. It must be at least 10 cm from the wall and away from flammable substances.InterferenceAvoid large temperature fluctuations, direct sunlight and vibrations. These conditions can distort measurements and micrographic images.In warm and warm-damp climatic zones, the individual components require special care in order to prevent the build-up of fungus.Use in clean roomsLeica E Series microscopes can be used in clean rooms without any problems.Handling electrical componentsNever install any other plug (NEMA 5-15P) or unscrew any mechanical components unless expressly instructed to do so in the instructions. The microscope must be connected to a grounded outlet.Always position the microscope so that you can disconnect it from the power supply at any time. The power cable is provided as the power disconnect device.Instructions for Use
Leica E Series User Manual Safety and Use 12Intervention from the userThe microscopes of the E Series contain no components that can be main-tained or repaired by the user. Exceptions are mentioned specifically in this manual. Unless otherwise specified in this manual, repair and service work may be carried out by authorized Leica technicians only.Unauthorized alterations to the instru-ment or noncompliant use shall void all rights to any warranty claims.MaintenanceLeica E Series microscopes are basically maintenance-free. To ensure that it always operates safely and reliably, we recom-mend that you take the precaution of contact-ing the responsible service organization. Here you can arrange for periodic inspections and conclude a maintenance contract (recom-mended).AccessoriesOnly those accessories may be used that are listed in this user manual or for which safe use has been confirmed by Leica Microsystems.Danger of infectionDirect contact with eyepieces is a poten-tial transmission method for bacterial and viral infections of the eye. The risk can be kept to a minimum by using personal eyepieces for each individual or detachable eyecups. Dangers during useThe microscope illumination is in the exempt group (risk group 0) according to EN 62471:2008 when used according to its intended use.•Never look directly into the LED beam of the illumination equipment – either with or without optical instruments – as this increases the risk class. Failure to observe this notice poses a risk of eye damage.Instructions for Use (Continued)
Leica E Series User Manual Leica ES2 13Leica ES2
Leica E Series User Manual Leica ES2 14Overview: Leica ES212357641. 10× fixed eyepieces for eyeglass wearers2. Carrying handle3. Two-level magnification changer 10× and 30×4. Focusing drive5. Integrated LED incident light illumination6. Integrated LED transmitted illumination7. Switch for incident and transmitted light
Leica E Series User Manual Leica ES2 15Built-in carrying deviceAll Leica E Series microscopes are equipped with a carrying handle for convenient and safe transport.Setting up the microscope1. Place the microscope on a flat tabletop.2. Connect the power supply to a grounded socket.Transport, Placement and StoragePacking the device away1. After use, wind up the cable as shown in the illustration.2. Store the instrument so that it is protected against dust.
Leica E Series User Manual Leica ES2 16Setting the eye distance1. Look into the eyepieces.2. Hold the eyepieces with both hands. Push the eyepieces together or separate them until you see a circular image.Note for eyeglass wearersIf you wear glasses, fold the eyecups back – otherwise, fold the eyecups forwards.The Correct Interpupillary DistanceThe ideal imageThe interpupillary distance is correctly set if you see a single circular image field when looking at a specimen.You may need a short time to become familiar with using the instrument. Not to worry – after a little while, it will become automatic.
Leica E Series User Manual Leica ES2 17Iluminating the specimen1. Position a specimen in the middle of the baseplate.2. Switch the two LED illuminators on or off as desired.After switching off, the LEDs remain lit for about another 4 seconds.Tips on use OUse incident light for plastic, opaque objects. OUse transmitted light for transmitted light specimens or transparent objects. OUse the combination of both LED illumina-tors for partially transparent objects.Using the LED Illuminator
Leica E Series User Manual Leica ES2 18Focusing raises and lowers the stereo-microscope using the focusing drive. The specimen is brought into sharp focus as soon as it is in the focal point of the objective.The focusing drive can be operated either left- or right-handed.Focusing1. Set the magnification changer to the lowest level (10).2. Set the working distance to approx. 100mm for coarse focusing.3. Set the magnification changer to the second level (30).4. Use the focusing drive once again for fine focus.After you have adjusted the image sharpness at high magnification, it is maintained even if you switch to a lower magnification (parfocal).FocusingFocusing drive torque
Leica E Series User Manual Leica ES2 19Magnification DisplayYou can read the configured magnifica-tion on the right-hand rotary knob of the Leica ES2. The additional magnification provided by the eyepieces is already incorpo-rated in the scale.
Leica E Series User Manual Leica ES2 20Adjusting the Resistance of the Focus DriveAdjusting the resistanceIs the focusing drive too easy or too difficult to adjust? No problem – the resistance can be adjusted individually depending on your personal preferences:1. Grip the drive knobs with both hands and turn them towards each other until the desired resistance is reached during focusing.
Leica E Series User Manual Leica EZ4 and Leica EZ4 W 21Leica EZ4 and Leica EZ4 W
Leica E Series User Manual Leica EZ4 and Leica EZ4 W 22Overview: Leica EZ412357641. 10× fixed eyepieces for eyeglass wearers2. Carrying handle3. Magnification changer4. Focusing drive5. Integrated LED incident light illumination6. Illumination control7. Integrated LED transmitted illumination
Leica E Series User Manual Leica EZ4 and Leica EZ4 W 23Instrument Variant: Leica EZ4 W12367109851. 10× fixed eyepieces for eyeglass wearers2. Integrated digital camera with SD card slot3. Mode selection panel4. Carrying handle5. Magnification changer6. IR receiver for optional remote control7. Focusing drive8. Integrated LED incident light illumination9. Illumination control10. Integrated LED transmitted illumination4
Leica E Series User Manual Leica EZ4 and Leica EZ4 W 24Built-in carrying deviceAll Leica E Series microscopes are equipped with a holder for convenient and safe transport.Setting up the microscope1. Place the microscope on a flat tabletop.2. Connect the power supply to a grounded socket.Transport, Placement and StoragePacking the device away1. After use, wind up the cable as shown in the illustration.2. Store the instrument so that it is protected against dust.
Leica E Series User Manual Leica EZ4 and Leica EZ4 W 25The Correct Interpupillary DistanceSetting the eye distance1. Look into the eyepieces.2. Hold the eyepieces with both hands. Push the eyepieces together or separate them until you see a circular image.Note for eyeglass wearersIf you wear glasses, fold the eyecups back – otherwise, fold the eyecups forwards.The ideal imageThe interpupillary distance is correctly set if you see a single circular image field when looking at a specimen.You may need a short time to become familiar with using the instrument. Not to worry – after a little while, it will become automatic.
Leica E Series User Manual Leica EZ4 and Leica EZ4 W 26Automatic shutoffIf the illumination is not operated for 60 minutes, it switches off automatically. The illumination can be reactivated by pressing a button on the membrane keypad. LED illumination1. Position a specimen in the middle of the baseplate.2. Switch the two LED illuminators on or off as desired: OUse incident light for plastic, opaque objects OUse transmitted light for transmitted light specimens or transparent objects. OUse the combination of both LED illumina-tors for partially transparent objects.For a detailed description of the illumina-tor, refer to page 28.Switching on the LED Illuminator
Leica E Series User Manual Leica EZ4 and Leica EZ4 W 27Disabling the automatic shutoffTo disable the automatic shutoff, follow these steps:1. Switch the microscope off.2. Press and hold the and buttons on the light control unit simultaneously while you switch on the microscope.The LEDs flash twice slowly and then remain switched on. The automatic shutoff is now disabled.Controlling the Automatic ShutoffAutomatic shutoffThe factory settings of the EZ4 models switch the instrument off automatically after one hour without user interaction. This automatic shutoff helps to conserve the light-emitting diodes and save energy. Enabling the automatic shutoffTo re-enable the automatic shutoff, follow these steps:1. Switch the microscope off.2. Press and hold the and buttons on the light control unit simultaneously while you switch on the microscope.The LEDs flash three times slowly and then remain switched on. The automatic shutoff is now re-enabled.
Leica E Series User Manual Leica EZ4 and Leica EZ4 W 28Three-level incident light illuminationThe incident light illumination is made up of 5 LEDs. Two, three or five diodes are switched on depending on the level. Accord-ingly, the angle of incidence of the light beam on the object is switched from high to low.Illumination TypesPress 1×Five diodes: greatest brightness for incident light objectsPress 2×Top three diodes: shadow-less uniform lighting for objects with highly uneven surfacesPress 3×Two diodes: side light emphasizes structures and increases the contrastPress 1×Switch on transmitted light for transparent objects and transmitted light specimensPress 4×Incident light offRegulating brightnessThe brightness is adjusted using the and keys and affects all switched-on LEDs.
Leica E Series User Manual Leica EZ4 and Leica EZ4 W 29FocusingFocusing raises and lowers the stereo-microscope using the focusing drive. The specimen is brought into sharp focus as soon as it is in the focal point of the objective.The focusing drive can be operated either left- or right-handed.Focusing1. Set the magnification changer to the lowest level (8).2. Set the working distance to approx. 100mm for coarse focusing.3. Set the magnification changer to the second level (35).4. Use the focusing drive once again for fine focus.After you have adjusted the image sharp-ness at high magnification, it remains constant even if you switch magnification levels (parfocal).Focusing drive torque
Leica E Series User Manual Leica EZ4 and Leica EZ4 W 30Magnification DisplayYou can read the configured magnifica-tion on the right-hand rotary knob of the Leica EZ4 models. The additional magnification provided by the eyepieces is already incorpo-rated in the scaleLeica EZ4 models with open tubesOn the Leica EZ4 model without eyepieces, only the magnification factors of the magnification changer are indicated: 0.8, 1, 1.25, 1.6, 2, 2.5, 3, 3.5.Different total magnifications are possi-ble depending on which eyepieces are used with the Leica EZ4. The magnification of the eyepieces is indicated on their outer surfaces, e.g. 10×/20, 16×/16 or 20×/12.
Leica E Series User Manual Leica EZ4 and Leica EZ4 W 31Adjusting the Resistance of the Focus DriveAdjusting the resistanceIs the focusing drive too easy or too difficult to adjust? No problem – the resistance can be adjusted individually depending on your personal preferences:1. Grip the drive knobs with both hands and turn them towards each other until the desired resistance is reached during focus-ing.
Leica E Series User Manual Eyepieces (Only for Leica EZ4) 32Eyepieces (Only for Leica EZ4)
Leica E Series User Manual Eyepieces (Only for Leica EZ4) 33Changing eyepiecesVarious eyepieces are available for the Leica EZ4, allowing the stereomicroscope to be adapted even better to the specific require-ments.The following eyepieces are available for selec-tion: OEyepieces replaceable, fixed or adjustable: 10×/20, 16×/16 or 20×/12 OEyepieces for eyeglass wearers 10×/20B and 16×/15B, fixed and adjustable The interchangeable eyepieces also offer the following advantages: OThe dioptric range is adjustable from +5 to –5 OMeasurement graticules can be used, see page 37 and the following.Changing Eyepieces (Only for Leica EZ4, Open Version)Adjustable eyepieces for eyeglasses with straight eyecups
Leica E Series User Manual Eyepieces (Only for Leica EZ4) 34Eyepieces and Eyecups (Only for Leica EZ4, Open Version)Eyeglass eyepiece with adjust-able diopter and straight eyecupsEyepiece for users who do not wear eyeglasses with adjustable diopter and inclined eyecupFixed eyepiece with inclined eyecupEyecupsThe eyecups can be replaced.Risk of infectionDirect contact with eyepieces is a poten-tial transmission method for bacterial and viral infections of the eye. The risk can be minimized by the use of individual eyecups.
Leica E Series User Manual Eyepieces (Only for Leica EZ4) 35Dioptric correctionThe adjustable eyepieces for the Leica EZ4 are also available with built-in diop-tric correction, allowing the microscope to be used without glasses even by those with vision problems. The correction comprises a maxi-mum of ±5 diopter settings.Users with normal vision do not need adjust-able eyepieces. An adjustable eyepiece is suffi-cient for mild shortsightedness. If vision is extremely poor or when graticules are used, two adjustable eyepieces are needed. When the diopters are regulated exactly, the sharpness is constant over the entire magnifi-cation range (=parfocal).Once the diopters have been set, the procedure does not have to be repeated. Simply restore your diopter values on the eyepiece before beginning work with the stereomicroscopeAdjusting1. Set the dioptric correction for both eyepieces to "0".2. Select the lowest magnification and focus on a flat specimen.3. Select the highest magnification and read-just the sharpness.4. Select the lowest magnification again, but do not look into the eyepieces.5. Carry out steps 6 to 8 for each objective individually. 6. Rotate the eyepiece counterclockwise in the "+" direction as far as it will go (+5 diopter settings).Dioptric Correction (Only for Leica EZ4, Open Version)
Leica E Series User Manual Eyepieces (Only for Leica EZ4) 36Dioptric Correction (Continued)7. Look into the eyepiece.8. Slowly rotate the eyepiece individually in the "–" direction until the eye sees the object sharply imaged. 9. Select the highest magnification and refocus if necessary.Now, if you adjust the magnification from the highest to the lowest level, the specimen is always brought into sharp focus. If not, repeat the process.
Leica E Series User Manual Eyepieces (Only for Leica EZ4) 37Graticules (Only for Leica EZ4, Open Version)GraticulesThe transparent graticules can be inserted in the adjustable eyepieces of the Leica EZ4. They enable easy measurements at the specimen.For equipment with a graticule in one eyepiece, two adjustable eyepieces are needed. Since one eye is focussed on the eyepiece with the graticule, it needs a second, adjustable eyepiece so that it can be aligned optically with the first.Available eyepiece insertsGraticules 10 mm/ 0.1 mm 5 mm/ 0.1 mm 5 mm/ 0.05 mm 100 Div / 0.002" 100 Div / 0.001" 150 Div / 0.0005"Stage micrometers 50 mm (0.1 / 0.01 mm graduation)1" (0.001" graduation)Crosshairs Crosshairs
Leica E Series User Manual Eyepieces (Only for Leica EZ4) 38Variant 2: 16× eyepiecesOn the 16× adjustable eyepieces, the insert is fixed to the eyepiece by a screwed connection.1. Screw the insert out counterclockwise.Variant 1: 10× and 20× eyepiecesThe 10× and 20× adjustable eyepieces include an insert at the bottom with a snap mount.1. Gently press the two small tabs on the snap mount together and pull out the insert.Graticules: PreparationCare instructionsThe graticule must always be clean and dust-free! Never touch the graticule with bare hands! Always hold the graticule by the edges!
Leica E Series User Manual Eyepieces (Only for Leica EZ4) 39Inserting the graticules is the same for all eyepiece types.1. Place the insert with the knurled side on the work surface.2. Grasp the graticule so that you see the scale in laterally correct form.3. Carefully take hold of the edge of the grati-cule and push it sideways into the holder in the insert. Make sure that both plastic holders snap into place.4. Put the insert back into the eyepiece. Depending on the variant, you have to either press or twist the insert into place.5. Insert the eyepiece into the tube.6. Align the graticules by turning the eyepiece in the tube.Inserting the Graticules
Leica E Series User Manual Photography Using the Leica EZ4 W 40Photography Using the Leica EZ4 W
Leica E Series User Manual Photography Using the Leica EZ4 W 41Thank you for purchasing the Leica EZ4 W by Leica Microsystems. The special design of the Leica EZ4 W makes it a universal, highly versa-tile tool for viewing microscopic specimens and capturing still images or video.Introduction
Leica E Series User Manual Photography Using the Leica EZ4 W 42Your Leica HD camera has many possibili-ties for various applications and condi-tions of use. Below are some basic concepts to help you get the most performance for your application.Modes of use1. SD mode Ocapture onto SD card Ouse remote control2. WiFi mode OWireless connection to wireless device (tablet, phone, PC)3. USB mode OCabled connection to PC4. Ethernet mode OCabled connection to external network5. Capture button OCaptures image onto the SD card The BasicsNote direct connection to an HD display is possible in all modes. Aspect ratio is 16:9 except in USB mode where the aspect ratio is 4:3.1 3245
Leica E Series User Manual Photography Using the Leica EZ4 W 43Leica EZ4 W: Overview1. LED status indicator OBlinking red means starting up OGreen means ready2. Capture button for SD card capture3. Mode selection panel 4. SD card slot1243
Leica E Series User Manual Photography Using the Leica EZ4 W 441. HDMI for connecting the provided HDMI cable2. Ethernet socket for connecting an ethernet cable (cable not provided)3. USB for connecting the provided USB cableFrontInfrared window: Receives the signal from the optional remote control.Leica EZ4 W: Overview (Continued)1 32
Leica E Series User Manual Photography Using the Leica EZ4 W 45UnpackingIf you ordered the handheld remote, you received the remote with batteries.The contents include CD with imaging software, HDMI cable, and USB cable.Please do not discard any of the packing materials. They should be used for safely storing and transporting the instrument should the need arise.
Leica E Series User Manual Get Set! 46Get Set!
Leica E Series User Manual Get Set! 4710× eyepieces have a fixed circular field of view of 18 mm or more diameter. The Leica EZ4 W’s field of view, however, is rectan-gular with a diagonal of 16.4 mm to ensure an even field on the digital image. As a result, the image field of the camera is smaller than the object field seen through the eyepieces.Installing and connecting1. Insert the provided CD into your computer, and follow the instructions to load the soft-ware.2. Insert the provided USB cable into the camera’s USB port, and attach the other end of the cable to a USB 2.0 port of your computer.3. Switch on the power of the EZ4 W micro-scope.4. The status light will blink red. Wait until the status light is green and one of the mode button's is also green. This may take approximately 30 seconds.USB Mode – Capturing and Adjusting Images Using a ComputerLASDVD
Leica E Series User Manual Get Set! 485. If a mode button other than USB mode is lit green, then press the USB mode button and wait until both the status LED and the USB mode button is green.6. You are ready to launch the Leica software. Follow the software instructions to adjust and capture an image.7. While in USB mode, you can connect the provided HDMI cable from the camera to an HD monitor or HD projector to share the image. Note the image aspect ratio while in USB mode is 4:3. To avoid distortion if your projector or monitor is set to a 16:9 aspect ratio, you will need to adjust the aspect ratio of the projector or monitor to 4:3 using the controls of the projector or monitor.USB Mode – Capturing and Adjusting Images Using a Computer (Continued)Note the image resolution on the HD projector or monitor is limited to the live image resolution setting in the software.4:3 aspect ratio 4:3 aspect ratio image on a screen set to an aspect ratio of 16:9
Leica E Series User Manual Get Set! 49In order to use this mode, you must have an SD card (not provided) with free memory on it. If the SD memory card is full, the capture button LED will not light green and you will not be able to capture any more images.Attaching the power cable1. Switch on the power to the EZ4 W micro-scope.2. The status light will blink red. Wait until the status light is green and one of the mode button's is also green. This may take approximately 30 seconds.SD Mode – Capturing Images Without a Computer or Without a Wireless Device3. If a mode button other than SD mode is lit green, then press the SD mode button and wait until both the status LED and the SD mode button is green.4. Insert the SD memory card into the slot on the side of the Leica EZ4 W until the card clicks into place.
Leica E Series User Manual Get Set! 50SD Mode – Capturing Images Without a Computer or Without a Wireless Device (Continued)The capture button LED will turn green. The Leica EZ4 W is now ready to capture images onto the SD memory card.Capturing an image•It is critical to properly set the micro-scope eyepieces’ diopters to ensure that the images on the SD memory card are in focus when the image is in focus through the micro-scope’s eyepieces. Refer to the microscope’s user manual for proper diopter setting. Focus the microscope at high magnification, then change to the magnification at which you wish to capture the image. Do not refocus the micro-scope. You can also use the HDMI output and an HD display (not provided) as a focusing aid. 5. To capture an image onto the SD memory card, lightly press the capture button, located on the side of the Leica EZ4 W camera. OYou will hear a beep to acknowledge the button was pressed. OThe status LED flashes red while the image is being captured. OYou will hear another beep when the Leica EZ4 W has completed capturing the image and the status LED will return to steady green.The image is now saved on the SD card. Images which are on the SD card can be viewed using the optional remote control. See "Camera Remote Control" on page 54.6. While in SD mode, you can connect the provided HDMI cable from the camera to an HD monitor or HD projector to share the image.
Leica E Series User Manual Get Set! 51Return to factory settingsPress and hold the service button for 5seconds. Wait for the beep. The camera will now reset to factory settings.Adjusting Settings While in SD ModeSetting a new white balanceMake sure that there is no sample in the beam path and that a standard illumina-tion is available when you reset the camera.Press and hold the capture button for 5 seconds. Wait for the beep. A new white balance is set.
Leica E Series User Manual Get Set! 52WiFi Mode – Capturing and Adjusting Images Using a Wireless Device 1. Switch on the power to the Leica EZ4 W microscope. 2. The status light will blink red. Wait until the status light is green and one of the mode button's is also green. This may take approximately 30 seconds.3. If a mode button other than WiFi mode is lit green, then press the WiFi button and wait until both the status LED and the WiFi mode button is green.4. Follow the directions of your WiFi view-ing device to connect to the inter-nal WiFi network of the camera. WiFi Password: leicamicro5. Open the appropriate app on your mobile device or appropriate software on your wireless enabled PC for viewing, capturing, and adjusting the camera image.
Leica E Series User Manual Get Set! 53Ethernet Mode – Capturing and Adjusting Images Using a Network1. Connect one end of an ethernet cable (not provided) to the camera and the other end of the cable to the ethernet connection in your facility.2. Switch on the power to the Leica EZ4 W microscope.3. The status light will blink red. Wait until the status light is green and one of the mode button's is also green. This may take approximately 30 seconds.4. If a mode button other than ethernet mode is lit green, then press the ethernet button and wait until both the status LED and the ethernet LED mode button is green.5. Follow the directions of your network compatible device to connect to your facili-ties network and select the camera. 6. Open the appropriate app on your mobile device or appropriate software on your wireless enabled PC for viewing, capturing, and adjusting the camera image.
Leica E Series User Manual The Camera Remote Control (Optional) 54The Camera Remote Control (Optional)The remote control is designed to work only when the camera is in SD mode and when the camera is used in combination with an HD display.The only function which works in any mode is the "Info" function which shows details about the camera.
Leica E Series User Manual The Camera Remote Control (Optional) 55The remote control can only work in SD mode except for the "i" button which will display information about the camera on an HD display. The remote must be pointed at the IR receiver of the camera without obstructions.1. Start/stop video recording2. Save still image to SD card3. Stop/continue live image, pause/play movie clip4. View files on SD card5. Move to previous image on SD card6. Move to next image on SD card7. Brightness adjustment up or down8. Pairing button9. Show/hide information box10. White balance set button11. Call up the camera menu12. Arrow keys for navigation13. OK to confirm1 3524678 9Optional Remote Control10 1112 137
Leica E Series User Manual The Camera Remote Control (Optional) 56Viewing Images and Movies With the Optional Remote ControlIf you have purchased the optional remote, the Leica HD camera shows images and movie clips directly on an HD moni-tor.1. Thumbnail view (first press) Oup/down/left/right  select thumbnail O Show image in full screen or playback movie clip Onext image / previous image O  Play movie clip Oleft /right  fast forward / rewind2. To return to live view press .Thumbnail viewPlayback / fullscreen view
Leica E Series User Manual The Camera Remote Control (Optional) 57The remote control can only work when the camera is in SD mode.Opening and closing the camera menu1. Point the remote control towards the camera.2. Press the key on the remote control to show the camera menus on the monitor.3. Press the  and  buttons to select a menu item.4. Press the button to confirm a menu item.5. Press the button again to hide the camera menus.Calling up the Camera Menu
Main menu Sub menu colorLeica E Series User Manual The Camera Remote Control (Optional) 58Enabling automatic white balance1. Press the button on the remote control.2. Call up the "COLOR" entry.3. Set the value for "WB MODE" to "AUTO".4. Press the button to exit the menu.The values for "RED" and "BLUE" cannot be adjusted if the "WB MODE" setting is set to "AUTO". COLORCOLOR (Automatic White Balance) EXPOSURE RESOLUTION SETUP CAMERA SETUP ETHERNET SETUP WIFI SET WB WB MODE RED LEVEL BLUE LEVEL BLACK LEVEL HUE PRESS OK AUTO SATURATIONThe "COLOR" function makes it possible to adapt the camera chip to the ambient light so that color-neutral images can be acquired.
Leica E Series User Manual The Camera Remote Control (Optional) 59The "COLOR" function makes it possible to adapt the camera chip to the ambient light so that color-neutral images can be acquired.Adjusting the white balance manually (recommended)1. Move the specimen out of the field of view so that only the illumina-tion is seen.2. Press the button on the remote control.3. Call up the "COLOR" entry.4. Set the "WB MODE" to "MANUAL".5. If necessary, correct the values for "RED" and "BLUE" until the image shows a uniform gray area.6. Press the button to exit the menu.COLOR (Manual White Balance)Main menu Sub menu color COLOR EXPOSURE RESOLUTION SETUP CAMERA SETUP ETHERNET SETUP WIFI SET WB WB MODE RED LEVEL BLUE LEVEL BLACK LEVEL HUE PRESS OK MANUAL SATURATION
Leica E Series User Manual The Camera Remote Control (Optional) 60Automatic exposure1. Press the button on the remote control.2. Call up the command for automatic exposure (see diagram at the left).3. Set the value for "EXP MODE" to "AUTO".4. Correct the values for "BRIGHTNESS" and "GAMMA" until you obtain the desired results for the image.Manual exposure1. Press the button on the remote control.2. Call up the command for manual exposure (see diagram at the left).3. Set the value for "EXP MODE" to "MANUAL".4. Correct the values for "EXPOSURE", "GAIN" and "GAMMA" until you obtain the desired results for the image.EXPOSUREMain menu Sub menu exposure COLOR EXPOSURE RESOLUTION SETUP CAMERA SETUP ETHERNET SETUP WIFI EXP MODE EXPOSURE GAIN BRIGHTNESS GAMMA AUTO Main menu Sub menu exposure COLOR EXPOSURE RESOLUTION SETUP CAMERA SETUP ETHERNET SETUP WIFI EXP MODE EXPOSURE GAIN BRIGHTNESS GAMMA MANUAL
Leica E Series User Manual The Camera Remote Control (Optional) 61The "RESOLUTION" menu enables you to define the resolution for the live image, still image capture and video capture individually. This provides optimum results in every situation.LIVE (resolution of the live image)Defines the resolution of the live image. CAPTURED (resolution of the captured image)Defines the resolution of the captured image on the SD card.MOVIE (resolution of videos)Indicates the resolution of the captured videos on the SD card.RESOLUTIONMain menu Sub menu resolution COLOR EXPOSURE RESOLUTION SETUP CAMERA SETUP ETHERNET SETUP WIFI LIVE CAPTURED MOVIE HD108060 5.0MPJPG HD720MP4
Leica E Series User Manual The Camera Remote Control (Optional) 62The camera settings enable you to control internal parameters such as the date or time, reset the camera, etc. RESET CAMERAThis command resets the camera to the factory settings. All user informa-tion (such as white balance, resolution etc.) is reset.SET DATE TIMEThis command sets the date and time as well as the format for display-ing them. The following formats are available for selection: YYMMDD – DDMMYY – MMDDYYFLIP IMAGESelect to flip image 180°.AUDIO/BEEP You can decide to have a tone from the camera for some functions.LANGUAGE Select the language of the On Screen Display.SELECTING IMAGE OVERLAYS (SELECT OVERLAY)Press to select another overlay. Press to activate the selected overlay and leave the menu. If you want to quickly display other overlays, press when the live image is displayed to show the next or previous respective overlay.CONFIGURING IMAGE OVERLAYS (CONFIG OVERLAY)Depending on the setting, this does not show an overlay, only shows the overlay in the live image or shows it in both the live image and the image capture. SETUP CAMERA (Camera Settings)Main menu Sub menu setup camera COLOR EXPOSURE RESOLUTION SETUP CAMERA SETUP ETHERNET SETUP WIFI RESET CAM SET DATE/TIME FLIP IMAGE PRESS OK PRESS OK 0° AUDIO/BEEP LANGUAGE SELECT OVERLAY CONFIG OVERLAY ENABLE ENGLISH NONE LIVE ONLY
Leica E Series User Manual The Camera Remote Control (Optional) 63This menu allows you to customize parameters relevant to working in Ethernet mode. Consult with your local IT expert for information relating to your local network.IP ADDRESSYou can decide if you want the IP address to be set by your network (Dynamic) or by you using the onscreen keyboard (Static).If you selected "STATIC" for the IP address you can input the following using the onscreen keyboard: OIP address OIP subnet mask OGateway IP address SETUP ETHERNETMain menu Sub menu Ethernet COLOR EXPOSURE RESOLUTION SETUP CAMERA SETUP ETHERNET SETUP WIFI IP ADDRESS IP ADDRESS STATIC IP SUBNET MASK DHCP Dynamic) GATEWAY IP ADDRESS Main menu Sub menu Ethernet COLOR EXPOSURE RESOLUTION SETUP CAMERA SETUP ETHERNET SETUP WIFI IP ADDRESS IP ADDRESS STATIC IP SUBNET MASK STATIC 192.168.1.200 255.255.255.0 GATEWAY IP ADDRESS 192.168.1.1
Leica E Series User Manual The Camera Remote Control (Optional) 64This menu allows you to customize parameters relevant to working in WiFi mode.INTERFACE  STAND ALONEThe camera is the access point.INTERFACE  NETWORKYour network provides the access point and the camera connects to your network by WiFi.NAME SSIDThis is the camera name which will appear. You can change the name using the onscreen keyboard.SECURITY OPTIONS  NONEThis means there is no password.SECURITY OPTIONS  WPA2PSK AESThis allows you to create a password.PASSWORDCreate a password using the onscreen keyboard.CHANNELWhen stand alone is selected as the interface, this allows the WiFi channel to be set automatically or by the user.ENABLE SSID BROADCASTWhen stand alone is selected as the interface, you can decide if you want the camera name to be seen as available to connect.WIFI PASSWORD: leicamicroSETUP WiFiMain menu Sub menu WiFi COLOR EXPOSURE RESOLUTION SETUP CAMERA SETUP ETHERNET SETUP WIFI INTERFACE NAME SSID SECURITY OPTIONS STANDALONE ICC50 W_2345 NONE PASSWORD 8 CHR CHANNEL AUTO ENABLE SSID BROADCAST YES
Leica E Series User Manual The Camera Remote Control (Optional) 65SCAN SSIDIf "OK" is selected, you will see a list of WiFi access points nearby.SETUP WiFi (Continued)Main menu Sub menu WiFi COLOR EXPOSURE RESOLUTION SETUP CAMERA SETUP ETHERNET SETUP WIFI INTERFACE NAME SSID SECURITY OPTIONS NETWORK ICC50 W_2345 NONE PASSWORD 8 CHR SCAN SSID OK
Leica E Series User Manual The Camera Remote Control (Optional) 66Pairing Cameras With RemotesPairingThe LeicaEZ4 W and the remote control can be paired and then only respond to each other. This can be helpful when using multiple cameras and remote controls.1. Press the button to start or end the process.In order to achieve successful pairing and avoid pairing by mistake, the second step must be performed within 4 seconds.If a "timeout" is displayed after 4 seconds, press the "Pair" button again to start the process.2. Press and hold the button on your remote you want to define as the pairing button until a corresponding confirmation is displayed on the HD monitor. All of the buttons except the button can be used for this.3. The microscope only responds to the remote control commands from this remote control.Resetting to factory setting1. Press the button to start the process.2. Press the button until a corresponding confirmation is displayed on the HD monitor.
Leica E Series User Manual Care, Transport, Contact Persons 67Care, Transport, Contact Persons
Leica E Series User Manual Care, Transport, Contact Persons 68General Instructions OKeeping all optical components clean is important for maintaining good optical performance. OAlways protect the microscope and camera with the plastic cover when the instrument is not in use. (Included in the delivery pack-age of the microscope.) Be sure power to the camera is off when the dust cover is in place. OIf any optical surface becomes badly coated with dust or dirt, it should be cleaned by blowing the surface with a syringe or brush-ing it off with a camel hair brush before attempting to wipe the surface clean. OOptical surfaces should be cleaned using a lint-free cloth, lens cloth or cotton swab moistened with a commercially available glass cleaner. Do not use alcohol. OAvoid excessive use of solvents. The lint-free cloth, lens cloth or cotton swab should be soaked with solvent, but not so wet that solvent runs over the lens. OProtect the microscope from moisture, fumes and acids and from alkaline, caustic and corrosive materials. Do not store chemicals in the vicinity. OProtect the microscope from oil and grease. Never grease or oil mechanical parts or sliding surfaces. OIt is advisable to enter a service agreement with Leica Service.TransportAlways use both hands to carry the microscope. There is a handle on the back of the microscope and an undercut in the front for this purpose.Contact address If your instrument no longer works perfectly, contact your Leica representative. You can find information on worldwide Leica representatives on the Leica website: www.leica-microsystems.com.Leica Microsystems (Switzerland) Ltd. Max Schmidheiny Strasse 201 CH-9435 Heerbrugg (Switzerland)Care, Transport, Contact Persons
Leica E Series User Manual Specifications 69SpecificationsDue to the technology of the camera, the speci- fications are changing regularly. Please visit our website www.leica-microsystems.com/ education for the latest brochure downloads to find the up to date specifications.
Leica E Series User Manual Specifications 70Camera SpecificationsOperating environmentFeature SpecificationTemperatureOperatingStorage+10°C to +40°C ( 50°F – 104°F)-20°C to +55°C ( -4°F – 131°F)Relative humidity (maximum)Operating/Storage 10 % to 90 % (noncondensing)Alitude (maximum)OperatingNonoperating 0 m to 2,000 m (0 ft to 6,561 ft)0 m to 10,668 m (0 ft to 35,000 ft)Pollution DegreeRating 2Ports and connectorsFeature SpecificationUSB one standard USB 2.0 (Typ B)Video one standard HDMI connector (Typ A)Network adapter one RJ-45 connectorMemory card reader supports up to 32 GBIntegrated powersupply100 V – 240 V ~ 50 / 60 Hz Automatic adjustment to local voltagePower consumption max. 30 W
Leica E Series User Manual Dimensions 71Dimensions
Leica E Series User Manual Dimensions 72Leica ES2
Leica E Series User Manual Dimensions 73Leica ES2 (Continued)
Leica E Series User Manual Dimensions 74Leica EZ4 W
Leica E Series User Manual Dimensions 75Leica EZ4 W (Continued)
Leica E Series User Manual Dimensions 76Leica EZ4, 10× Eyepieces
Leica E Series User Manual Dimensions 77Leica EZ4, 10× Eyepieces (Continued)
Leica E Series User Manual Dimensions 78Leica EZ4 without Eyepieces
Leica E Series User Manual Dimensions 79Leica EZ4, without Eyepieces (Continued)
13IDE10060EN_2 • Copyright © by Leica Microsystems (Schweiz) AG, CH-9435 Heerbrugg, 2015. Subject to modifications. LEICA and the Leica Logo are registered trademarks ofLeica Microsystems IR GmbH.www.leica-microsystems.comThe statement by Ernst Leitz in 1907, “With the User, For the User,” describes the fruitful collaboration with end users and driving force of innovation at Leica Microsystems. We have developed five brand values to live up to this tradition: Pioneering, High-end Quality, Team Spirit, Dedication to Science, and Continuous Improvement. For us, living up to these values means: Living up to Life.INDUSTRY DIVISIONThe Leica Microsystems Industry Division’s focus is to support customers’ pursuit of the highest quality end result. Leica Microsystems provide the best and most innovative imaging systems to see, measure, and analyze the microstructures in routine and research industrial applications, materials science, quality control, forensic science investigation, and educational applications.Leica Microsystems – an international company with a strong network of worldwide customer services:Active worldwide Tel. FaxAustralia ∙ North Ryde +61 2 8870 3500 2 9878 1055Austria ∙ Vienna +43 1 486 80 50 0 1 486 80 50 30Belgium ∙ Diegem +32 2 790 98 50 2 790 98 68Brazil ∙ São Paulo +55 11 2764-2411 11 2764-2400Canada ∙ Concord/Ontario +1 800 248 0123 847 405 0164Denmark ∙ Ballerup +45 4454 0101 4454 0111France ∙ Nanterre Cedex +33 811 000 664 1 56 05 23 23Germany ∙ Wetzlar +49 64 41 29 40 00 64 41 29 41 55India ∙ Mumbai +91 226 1880 200 226 1880 333Italy ∙ Milan +39 02 574 861 02 574 03392Japan ∙ Tokyo +81 3 5421 2800 3 5421 2896Korea ∙ Seoul +82 2 514 65 43 2 514 65 48Netherlands ∙ Rijswijk +31 70 4132 100 70 4132 109People’s Rep. of China ∙ Hong Kong +852 2564 6699 2564 4163 ∙ Shanghai +86 21 6039 6000 21 6387 6698Portugal ∙ Lisbon +351 21 388 9112 21 385 4668Singapore +65 6550 5999 6564 5955Spain ∙ Barcelona +34 93 494 95 30 93 494 95 32Sweden ∙ Kista +46 8 625 45 45 8 625 45 10Switzerland ∙ Heerbrugg +41 71 726 34 34 71 726 34 44United Kingdom ∙ Milton Keynes +44 800 298 2344 1908 577640USA ∙ Buffalo Grove/lllinois +1 800 248 0123 847 405 0164

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