Leica Biosystems Nussloch SPECTRACV01 HistoCore SPECTRA CV User Manual Leica SPECTRA CV Instructions for Use V1 2 RevA

Leica Biosystems Nussloch GmbH HistoCore SPECTRA CV Leica SPECTRA CV Instructions for Use V1 2 RevA

User Manual V1.2 Part 6

148 Version 1.2, Revision A Malfunctions and Troubleshooting81Fig. 115 11. Hold the elevator with one hand (→ Fig. 116-1), loosen the locking mechanism (→ Fig. 116-2) at the elevator and fold it back (→ Fig. 116-3).12. Continue to hold the elevator with one hand and remove the rack from the elevator with the other hand (→ Fig. 116-4). Make sure that no slides slip out of the rack.13. Now release the elevator and take the removed rack out of the instrument.1234Fig. 116 14. Close the locking mechanism of the elevator again (→ Fig. 117-1) and push the elevator a few centimeters downward (→ Fig. 117-2).
149HistoCore SPECTRA CVMalfunctions and Troubleshooting 812Fig. 117 15. Remove the needle from the parking location and insert it into the holder of the coverslip line. Make sure that the needle is positioned correctly.16. Close the hood.Note • After initializing the instrument again, the coverslip line can be used for processing again.• If the coverslip line continues to be defective, notify Leica Service. Do not continue to use the defective coverslip line in the meantime.8.3.3 Removing the rack from the lower area of the left elevator1. Moving the unload drawer into removal position (rescue position)2. Reach to the left elevator with your left hand through the unload drawer (or through the load drawer after the vessels have been removed).3. Press the rack downwards and out of the elevator (hold the holder from below using your little finger) and push it toward the back.4. The rack can also be removed upwards using the coverslip line and the elevator. To do so, please note the action as described in step 2.
150 Version 1.2, Revision A Malfunctions and Troubleshooting88.3.4 Removing the rack from the oven or from behind the ovenWarning • Danger of explosion! Easily flammable reagents in the oven• There is a potential for respiratory tract irritation through evaporating flammable reagents.• Caution! Hot surfaces! The oven door and the interior of the oven are hot. Avoid contact if possible to prevent burns.1. Open access to the oven (→ Fig. 118-1).2. Open the oven door (→ Fig. 118-2) fully upwards (→ Fig. 118-3), the magnet holds the oven door in place (→ Fig. 118-4). 3. If the rack is not attached to the gripper (→ Fig. 118-5), the rack can be removed from the oven (→ Fig. 118-6).WarningIt is possible that the mounting medium is not yet sufficiently dried. Because of this, remove the rack only with great caution to prevent sliding of the coverglasses on the slides.4. Store the rack safely outside the instrument. 5. Detach the oven door from the magnet carefully (→ Fig. 118-7) and close it (→ Fig. 118-8).WarningClose the oven door carefully to prevent crushing your hand.Note You must feel the oven door click into place in the lock.6. Finally, close the access to the oven (→ Fig. 118-1).
151HistoCore SPECTRA CVMalfunctions and Troubleshooting 816273845Fig. 118 8.3.5 Removal of the rack from the rotator1. Moving the unload drawer into removal position (rescue position).2. Insert your hand carefully into the instrument and remove the rack (→ Fig. 119-1) from the rotator (→ Fig. 119-2).
152 Version 1.2, Revision A Malfunctions and Troubleshooting812Fig. 119 8.3.6 Removal of the rack from the gripper above the rotator1. Moving the unload drawer into removal position (rescue position).2. Insert your hand carefully into the instrument and remove the rack from the gripper arm. To do so, grasp the handle and pull it forward, along with the rack, toward the load drawer.8.3.7 Removal of a rack from the transfer station of the HistoCore SPECTRA ST LIf a malfunction occurs in workstation mode while the transfer device of the HistoCore SPECTRA ST transfers a rack to the HistoCore SPECTRA CV using the transfer station, the user must check where the rack is located.1. Open the hood of the HistoCore SPECTRA ST.2. Check whether the rack is still visible from the transfer station (→ Fig. 120-1). 1Fig. 120
153HistoCore SPECTRA CVMalfunctions and Troubleshooting 83. If this is the case, push the sledge (→ Fig. 121-1) for the transfer station back into the HistoCore SPECTRA ST manually (→ Fig. 121-2) and remove the rack (→ Fig. 121-3) from the holder (→ Fig. 121-4).1234Fig. 121 4. Following this, remove the rack from the instrument and store it safely.5. After the instrument fault has been corrected, insert the rack in the load drawer of the HistoCore SPECTRA CV to start processing.8.4 Replacing main fusesWarningThe instrument should be shut down using the Main Switch and disconnected from the power supply in the case of a instrument failure. The main fuses can then be inspected.A suitable flat-head screwdriver must be used to prevent damage to the fuse holders.Beware of defective fuses! Potential risk of injury due to broken glass! Wear suitable protective clothing (safety goggles, cut-resistant gloves (→ P. 19 – 3.1 Standard delivery – packing list)).1. To do so, open the hood and unscrew the two fuse holders (→ Fig. 122-1) on the top of the right cover with a flat-head screwdriver2. Check the fuse being used for damage.
154 Version 1.2, Revision A Malfunctions and Troubleshooting81Fig. 122 3. Remove the defective fuse from the holder and replace it with a new fuse.4. Installation occurs in the reverse sequence of operations.
155HistoCore SPECTRA CVOptional Accessories and Consumables 99. Optional Accessories and Consumables9.1 Optional accessoriesDesignation Order No.Rack for 30 slides* (3 pcs. per package) 14 0512 52473Handle for rack for 30 slides* (yellow, 3 pcs. per package) 14 0512 52476Handle for rack for 30 slides* (light blue, 3 pcs. per package) 14 0512 52477Handle for rack for 30 slides* (dark blue, 3 pcs. per package) 14 0512 52478Handle for rack for 30 slides* (pink, 3 pcs. per package) 14 0512 52479Handle for rack for 30 slides* (red, 3 pcs. per package) 14 0512 52480Handle for rack for 30 slides* (green, 3 pcs. per package) 14 0512 52481Handle for rack for 30 slides* (black, 3 pcs. per package) 14 0512 52482Handle for rack for 30 slides* (gray, 3 pcs. per package) 14 0512 52483Handle for rack for 30 slides* (white, 3 pcs. per package) 14 0512 52484Reagent vessel, assembly, each consisting of 1 pc.: 14 0512 47086 Reagent vessel 14 0512 47081 Reagent vessel cover 14 0512 47085 Reagent vessel handle 14 0512 47084Label cover S 14 0512 53748Label cover, blank 14 0512 47323Waste tray 14 0514 49461Solvent container kit (2 pcs.) 14 0514 54195Prime bottle, assembly, consisting of: 14 0514 53931 Lab bottle, 150 ml 14 0514 56202 Screw cap 14 0478 39993 Prime bottle insert 14 0514 57251 28x3 mm O-ring 14 0253 39635Cleaning bottle 14 0514 57248Suction cup 14 3000 00403Rack storage rails for the unload drawer 14 0514 55967Active carbon filter set, consisting of: 14 0512 53772 Active carbon filter 14 0512 47131Exhaust air hose set, consisting of: 14 0514 54815 Exhaust air hose, 2 m 14 0422 31974 Hose clamp 14 0422 31973Cut-resistant gloves, size M, 1 pair 14 0340 29011
156 Version 1.2, Revision A Optional Accessories and Consumables9Designation Order No.Tool kit HistoCore SPECTRA CV, consisting of: 14 0514 54189 Screwdriver, 5.5x150 14 0170 10702 Leica brush 14 0183 30751 T16A fuse 14 6000 04696Fig. 123 Exhaust hose1 set, consisting of the following:• Exhaust hose of length: 2 m• Hose clampOrder No.: 14 0422 31974Fig. 124 Active carbon filter1 set, consisting of 2 pcs.Order No.: 14 0512 53772Fig. 125 Waste trayOrder No.: 14 0514 49461
157HistoCore SPECTRA CVOptional Accessories and Consumables 9Fig. 126 Needle cleaning container1 kit, consisting of 2 pcs.Order No.: 14 0514 54195Fig. 127 Reagent vesselAssembly, incl. reagent vessel coverOrder No.: 14 0512 47086
158 Version 1.2, Revision A Optional Accessories and Consumables9Fig. 128 Racks30 specimen slides (3 pcs. per package)Order No.: 14 0512 52473Fig. 129 Handle for racks30 specimen slides (3 pcs. per package)Color Order No.:• yellow 14 0512 52476• light blue 14 0512 52477• dark blue 14 0512 52478• pink 14 0512 52479• red 14 0512 52480• light green 14 0512 52481• black 14 0512 52482• gray 14 0512 52483• white 14 0512 52484Fig. 130 Prime bottleAssembly, consisting of:• Lab bottle, 150 ml• Screw cap• Prime bottle insert• 28x3 mm O-ringOrder No.: 14 0514 53931

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