Leica Biosystems Nussloch SPECTRACV01 HistoCore SPECTRA CV User Manual Leica SPECTRA CV Instructions for Use V1 2 RevA

Leica Biosystems Nussloch GmbH HistoCore SPECTRA CV Leica SPECTRA CV Instructions for Use V1 2 RevA

User Manual V1.2 Part 2

35HistoCore SPECTRA CVInstallation and Instrument Setup 412Fig. 15 4.6.2 Inserting the mounting medium bottle and prime bottle1. Open the hood.2. Grasp the bottle sledge on the handle (→ Fig. 17-1) and move it all the way forward.Inserting the prime bottle » Insert the prime bottle (→ Fig. 17-2) into the position provided for it (→ Fig. 4-11) in the bottle sledge.Inserting the mounting medium bottleNote • Before the insertion of a mounting medium bottle in the instrument, the expiration date (imprint on the packaging and on the bottle label (→ Fig. 16-3)) is to be checked. If the expiration date has been reached or exceeded, the mounting medium may no longer be used. Expired mounting medium is to be disposed of in accordance with the applicable on-site lab guidelines.• Opened mounting medium has a storage life of 14 days, within which it must be spent. We recommend making a note of the use-date on the bottle label.• Only original mounting medium that has been validated by Leica may be used (→ P. 160 – Consumables) in order to prevent instrument faults.WarningEnsure the correct assignment of the cannulas (→ Fig. 17-4) to the respective mounting medium bottles to prevent instrument faults. The cannula with the mark 1 is to be inserted into the mounting medium bottle in position 1 and the cannula with the mark 2 into the mounting medium bottle in position 2 of the bottle sledge (→ Fig. 17-3).
36 Version 1.2, Revision A Installation and Instrument Setup4Note The insertion of a mounting medium bottle in the coverslip line L1 is described below. The same procedures also apply for the coverslip line L2.1. Take the mounting medium bottle (→ Fig. 16) out of the packaging and remove the black plastic cap (→ Fig. 16-1).Note Do not dispose of the black cover cap. If a non-empty bottle of mounting medium is taken out of the instrument (e.g. in the event of transport), it can be closed up again using the black plastic cap and retained. However, while doing so, the expiration date of the opened bottle must be observed.Warning• Do not remove the white plastic seal (→ Fig. 16-2). It must remain on the bottle.• Before the initial insertion of the cannulas, check whether the caps on the ends of the cannulas have been removed. 1234Fig. 16 2. Insert the mounting medium bottle into the recess L1 and make sure that the RFID chip (→ Fig. 16-4) is seated in the notch of the recess (→ Fig. 17-5).
37HistoCore SPECTRA CVInstallation and Instrument Setup 4124433365Fig. 17 3. Carefully insert the cannula (→ Fig. 17-4) with the label 1 into the opening of the mounting medium bottle until it clicks into place. This process involves puncturing the white protective membrane.WarningPuncturing the white protective membrane briefly requires an increase in force. Therefore, proceed carefully to avoid slipping from your hand. 4. Repeat the process for bottle L2.5. Slide the bottle sledge (→ Fig. 17-1) back until you feel it click into place.6. Close the hood. 7. The mounting medium bottles that are inserted are detected by the instrument software and the Module Status (→ Fig. 23) is updated.Note The software identifies the insertion of the new bottles and informs the user that an Extended Prime is necessary for both coverslip lines (→ P. 126 – 7.3.2 Extended Prime). Only afterwards is the instrument ready to start.
38 Version 1.2, Revision A Installation and Instrument Setup44.6.3 Prepare the reagent vessel, fill it and insert it into the load drawerTo ensure that everything runs smoothly in the instrument, observe the following notes and proceed as follows.Attaching the reagent vessel handle: » Check whether the reagent vessel handle is correctly attached to the reagent vessel. If this is not the case, fasten the handle according to (→ Fig. 18).Fig. 18 12Correct filling of reagent vessels:Note • The reagent vessel must be filled with a reagent which is both compatible with the HistoCore SPECTRA ST and with the mounting medium used (→ P. 33 – 4.6 Refilling consumables).• Reagent vessels must always be filled outside the instrument.• Make sure that the reagent vessel handle is not bent. During the removal of the reagent vessel, if the handle is bent out of shape, it may become unfixed from the reagent vessel and the reagent that is filled could be spilled.WarningThe safety notes for reagent handling must be observed!
39HistoCore SPECTRA CVInstallation and Instrument Setup 4Correct fill level of the reagent vesselsNote • When filling the reagent vessels, observe the fill level marks inside the reagent vessels.• Use the label covers contained in the (→ P. 19 – 3.1 Standard delivery – packing list) to label the reagent vessels in the load drawers. • The label covers with the letter S printed on them (→ Fig. 19-2) indicate to the user that the reagent vessel has been filled with a solvent. • Use the non-labeled covers only in cases where the reagent vessels are empty (e.g. after shutting down the instrument).A sufficient fill level is ensured if the level of the reagents is between the maximum (→ Fig. 18-1) and the minimum (→ Fig. 18-2) fill level mark.Inserting the reagent vessels into the load drawer:1. To insert the reagent vessels, press the drawer button for the load drawer (→ Fig. 2-4).2. The load drawer opens.3. Remove the reagent vessel and fill it with a HistoCore SPECTRA ST compatible solvent outside the instrument in accordance with the safety instructions.4. Then insert the filled vessel back into the load drawer (→ Fig. 19-1).5. Close the load drawer by pressing the drawer button again.Fig. 19 12
40 Version 1.2, Revision A Operation55. Operation5.1 User interface – overviewThe HistoCore SPECTRA CV is programmed and operated using a color touchscreen. If there is no processing in progress, the screen displays the following main window (→ Fig. 20) after switching on.1523467891011Fig. 20 1Status bar 7Process status display menu2Process status display 8Module status menu3Consumables status display 9Parameter sets menu4Load drawer status display 10 Settings menu5Unload drawer status display 11 User Settings menu6Main menu (→ P. 46 – 5.6 Main menu overview)
41HistoCore SPECTRA CVOperation 55.1.1 Grayed out function keysNote No changes can be carried out in the settings during processing or while there are racks in the instrument (load drawer, unload drawer, oven, coverslip line). Corresponding function keys are grayed out and therefore deactivated.However, at all times it is possible to:• Refill consumables (mounting medium, coverglass)• Run the cleaning programs Quick Prime, Extended Prime and Clean the Hose System,• Switch from Standard User Mode to the Supervisor Mode.
42 Version 1.2, Revision A Operation55.2 Elements of the status display110261137124859Fig. 21 1Current date2If the remaining service life for the battery is about 3 months or less, this symbol appears on the status bar instead of the date. A notification is sent to the user at the same time.3If the service life of the battery has expired, this symbol appears on the status bar instead of the date. A notification that the battery needs to be replaced by a Leica service technician is sent to the user at the same time.4If alarms and error messages are displayed during operation, this alarm symbol appears. Pressing this symbol allows the last 20 active messages to be viewed again.5If warnings and notes are displayed during operation, this notification symbol appears. Pressing this symbol allows the last 20 active messages to be viewed again.6Local time7The Process symbol indicates that processing is currently active and that a rack may still be in the unload drawer or a rack from the HistoCore SPECTRA ST is expected.8This symbol displays that the oven is active and is in the heating phase.9This symbol displays that the oven is active and is ready to operate.10 This symbol displays that the oven has been deactivated.11 The operation of the instrument in Supervisor Mode is displayed by this symbol. This mode provides additional operation and adjustment options for trained staff. Access to this mode is password-protected.12 This User symbol displays that the instrument is in user mode, which permits a simplified instrument operation without password.
43HistoCore SPECTRA CVOperation 55.3 Process status display• In the Process status display (→ Fig. 20-2), all racks in the process (→ Fig. 22-2) are displayed in color of the respective rack handle.• The status bar (→ Fig. 22-1) for the main window lists the parameter sets that can currently be started with the number and color assigned to the rack handles. These symbols are also visible if the daily Quick Prime (→ P. 125 – 7.3.1 Quick Prime) has not yet been carried out.Note Each ongoing processing is illustrated by a rack handle symbol. It is shown in the same color as the actual rack handle. Various information is displayed on the handle symbol (→ Fig. 22).1627345Fig. 22 1Parameter sets that can be started 5Progress display of processing2Racks in the process 6Estimated remaining time (hh:mm)3Parameter set number 7Time at end of process4Current position of the rack in the instrument: CV1/CV2 = coverslip line L1/L2, RED = rotator, O01/O02 = oven position 1/2
44 Version 1.2, Revision A Operation55.4 Consumables Management System (CMS)Note To call up this menu, press the Module Status menu (→ Fig. 20-8) button. The HistoCore SPECTRA CV has a Consumables Management System (CMS) that automatically monitors various levels and notifies the user of the following details:• Mounting medium designation (→ Fig. 23-1)• Remaining number of slides (→ Fig. 23-2) • Coverglass length (→ Fig. 23-3)• Remaining number of coverglass (→ Fig. 23-4)• The percentage display (→ Fig. 23-5) (→ Fig. 23-6) displays the consumption status of the mounting medium and the coverglasses in the respective left or right coverslip line. The colored portion of the bar lowers accordingly as usage continues.162345Fig. 23
45HistoCore SPECTRA CVOperation 55.5 Displaying the drawersThe lower area of the main window (→ Fig. 24) shows the status of the load drawer, the unload drawer and the consumables (coverglass and mounting medium).• The stations depicted by a directional arrow in the instrument (→ Fig. 24-1) symbolize the load drawer.• The center area (→ Fig. 24-2) displays the assigned and available positions in the unload drawer.• The right-hand area (→ Fig. 24-3) shows the state of the filled consumables (coverglass and mounting medium).• After closing the load drawers, the instrument automatically detects any inserted or removed racks.• The racks in both drawers are shown on the screen with the respective rack handle color (→ Fig. 24-1) (→ Fig. 24-2). Available positions are displayed as empty.1 2 3Fig. 24 Note The drawers can be opened when the drawer buttons (→ Fig. 2-4) and (→ Fig. 2-5) light up green.A drawer button is illuminated in red and cannot be opened in the following cases: • If there is a rack used by the instrument in the input or unload drawer,• If a rack is to be transferred from the HistoCore SPECTRA ST to the HistoCore SPECTRA CV, • If a rack is being turned in the rotator• If a rack is being inserted into the rack elevator or removed from the rack elevator.WarningFor manual loading of the HistoCore SPECTRA CV in workstation mode, the user is responsible for ensuring racks would be suitable for being transferred from the HistoCore SPECTRA ST to the HistoCore SPECTRA CV at the same time. The end times of the HistoCore SPECTRA ST have to be taken into account before loading manually since otherwise there can be delays in the HistoCore SPECTRA ST that can affect the staining result.
46 Version 1.2, Revision A Operation55.6 Main menu overviewThe Main menu (→ Fig. 20-6) is located on the left side of the display, which is divided as described below. This menu is visible in all submenus and allows switching to another submenu at any time.The Process Status Display (→ Fig. 20-7) displays the current status of all racks in process. Here, the respective handle of the rack is displayed in representational form with the respective color. This display shows the standard display.The Module Status (→ Fig. 20-8) displays an overview of the consumables filled for the respective coverslip line (mounting medium and coverglasses) and enables access to the cleaning and priming options menu for the two coverslip lines.The Parameter sets (→ Fig. 20-9) menu item is used to create and manage parameter sets.Basic settings can be configured in the Settings (→ Fig. 20-10) menu. The language version, date and time and other parameters can be adapted to local requirements here. The oven can be switched on or shut down.In the User Settings (→ Fig. 20-11) menu, a custom password can be set up to prevent modifications to the programs and reagent lists by unauthorized persons (Supervisor Mode). However, the instrument can be used without a password in the standard User Mode.5.6.1 Entry keyboardNote A keyboard appears (→ Fig. 25) for required entries (creating or entering a password). It is operated using the touchscreen.Note that the keyboard display depends on the configured language.
47HistoCore SPECTRA CVOperation 5162738 459Fig. 25 1Title bar2Input field3Delete the most recently entered character4Confirmation5Move cursor to left or right6Space key7Special character shift button (→ Fig. 26)8Cancel (entries are not saved!)9Upper and lowercase (pushing the button twice activates caps lock, indicated by the button turning red. Pressing again re-activates lowercase.)
48 Version 1.2, Revision A Operation5Special character keyboardFig. 26 Other special characters1. To enter a special character or umlaut, etc. not included in the special character keyboard (→ Fig. 26), hold the corresponding normal button on the keyboard.2. Example: Holding the standard "a" button brings up other selection options (→ Fig. 27).3. Select the required character from the new single-line keyboard by pressing it.Fig. 27 Note Number of characters for passwords: min. 4 to max. 16 characters.
49HistoCore SPECTRA CVOperation 55.7 User settingsThis menu can be used to configure the appropriate access level. A distinction is made between the following:• Standard user• Supervisor (password-protected)• Service technician (password-protected)Standard user:The standard user does not need a password and can use the completely configured instrument for all routine applications. It is not possible for this user group to modify programs and settings.Supervisor:The supervisor has the same access options as the standard user, but when the instrument is in sleep mode, they can also create, edit and delete parameter sets, change settings and perform the instrument setup functions. Therefore, supervisor access is password-protected.To activate supervisor mode, proceed as follows:1. Press the Supervisor button (→ Fig. 28-1).1 2Fig. 28
50 Version 1.2, Revision A Operation52. A keyboard (→ Fig. 29) is then displayed which can be used to enter the password.Fig. 29 13. The entry is finished by confirming with OK (→ Fig. 29-1) and the entered password is checked for validity. The current user status is displayed with the respective symbol in the status bar (→ Fig. 21) on the top right.Note The password configured at the factory should be changed during the initial setup.To change the supervisor password, proceed as follows:1. To change the password, press the Change Password button (→ Fig. 28-2) and enter the old password.2. Then, enter the new password twice using the keyboard and confirm with OK.Note A password must have at least 4 characters and may have up to 16 characters.Service technician:The service technician can access system files and carry out basic settings and tests.
51HistoCore SPECTRA CVOperation 5Note Access to this service area of the software is only possible for technicians trained by Leica and certified for this instrument type.5.8 Basic settingsThe Settings (→ Fig. 30-1) menu is opened by pressing the gear symbol (→ Fig. 30). Basic instrument and software settings can be configured in this menu.• Touching one of the displayed symbols, e.g. Language (→ Fig. 30-2), opens the corresponding submenu.12Fig. 30 Note The individual submenus are described in the following chapters.
52 Version 1.2, Revision A Operation55.8.1 Language settingsRequired access level: Standard user, supervisor• The language selection menu is displayed by pushing the symbol for the Language (→ Fig. 30-2). This menu contains an overview of all languages installed in the instrument and allows the desired display language to be selected.• Select the desired language and confirm by hitting Save.• The screen display and all information messages and labels are displayed immediately in the currently configured language.Note A supervisor or Leica service technician can add other languages (if available) by using Import (→ P. 62 – 5.8.7 Data management).5.8.2 Regional settingsRequired access level: Standard user, supervisorBasic format settings can be carried out in this menu.Date format:• Configure the date display (→ Fig. 31-1) to international, ISO or US format by pressing the corresponding radio button (→ Fig. 31-2).• The activated setting is labeled by a red border (→ Fig. 31-2). Time format:• The time display can be changed from a 24-hour display to a 12-hour display (a.m. = morning/p.m. = afternoon) using the slider (→ Fig. 31-3).• Pushing the Save (→ Fig. 31-5) button saves the settings.• If you do not want to apply the settings, press the Back (→ Fig. 31-4) button to return to the previous menu.
53HistoCore SPECTRA CVOperation 512345Fig. 31 5.8.3 Date and timeRequired access level: Standard user, supervisorThe current date (→ Fig. 32-1) and local time (→ Fig. 32-2) can be configured in this menu by rotating the individual rollers.• Pushing the Save (→ Fig. 32-4) button saves the settings.• If you do not want to apply the settings, press the Back (→ Fig. 32-3) button to return to the previous menu.
54 Version 1.2, Revision A Operation51 23 4Fig. 32 Note In the 12-hour display, a.m. (morning) and p.m. (evening) are displayed under the hour digits to enable a correct setting.The time and date settings cannot deviate more than 24 hours from the system time configured at the factory.5.8.4 Menu for alarm sounds – Error and signal soundsRequired access level: Standard user, supervisorIn this menu, audible note and error signals can be selected, the volume can be configured and functionality can be tested.The current setting for alarm and error sounds is displayed after calling up the menu.WarningAfter starting the instrument, an error sound is played. If this does not occur, the instrument may not be operated. This protects the specimens and the user. In this case, contact the responsible Leica service organization.
55HistoCore SPECTRA CVOperation 512345577668Fig. 33 Sound Type 1 - Note (→ Fig. 33-1)Signal sounds are issued if warning messages or notifications are displayed on the screen. You can select from a list of 6 sounds. To change the settings, press the Edit (→ Fig. 33-3) button. The Test (→ Fig. 33-5) button can be used to listen to the respective sound. The volume can be adjusted incrementally by turning the roller (0 to 9). Sound Type 2 - Error (→ Fig. 33-2)WarningThe user must stay within earshot during the operation in order to react immediately during instrument malfunctions.Error sounds are issued if an error message is displayed on the screen. This requires immediate intervention from the user. To change the settings, press the Edit (→ Fig. 33-4) button.
56 Version 1.2, Revision A Operation5• The volume setting for error sounds is adjusted using the Volume roller. Six different sounds are available for the alarm types. The Test (→ Fig. 33-5) button can be used to listen to the respective sound.• The volume can be adjusted incrementally by turning the roller.Note The acoustic error sounds cannot be disabled. The minimum configurable value for the volume is 2. The maximum value is 9.• Pushing the Save (→ Fig. 33-6) button saves the settings. The Cancel (→ Fig. 33-7) button is used to close the selection window without applying the settings.• Press the Back (→ Fig. 33-8) button to return to the Settings menu.5.8.5 Oven settingsRequired access level: Standard user, supervisorThe operating mode of the oven can be configured in the menu for oven settings (→ Fig. 34). The current oven settings are displayed after calling up the menu.Note • When starting the instrument, the oven is normally enabled and the Exclude Oven Step (→ Fig. 34-3) is disabled.• The oven heats up to approx. 35 °C. As soon as a rack has been coverslipped, the oven continues heating up to approx. 40 °C. The symbol in the status bar changes from the status "heating up" (→ Fig. 21-8) to the status "ready to operate" (→ Fig. 21-9). • The oven dries the cover slipped slides after completed processing for approx. 5 minutes. After the oven step has ended, the mounting medium is not completely dry. Treat the slides carefully during the removal from the rack to avoid a sliding of the coverglass.• The temperature setting of the oven (maximum 40 °C) and the dwell time of the finished coverslipped slides are both configured at the factory and cannot be changed by the user. • It can take up to 4 minutes for the oven to heat up!
57HistoCore SPECTRA CVOperation 554321Fig. 34 Shutting down the oven1. To shut down the oven, press the Oven off (→ Fig. 34-2) button.2. If the oven is shut down, this is indicated by the button (→ Fig. 34-2) being colored red/white.3. Apply the shutdown by pressing the Save (→ Fig. 34-5) button.4. Observe both of the following information messages (→ Fig. 35-1) and (→ Fig. 35-2) and acknowledge each with Ok.5. If you do not want to apply the settings, press the Back (→ Fig. 34-4) button to return to the previous menu without saving the changes.Fig. 35 12
58 Version 1.2, Revision A Operation5Note • If the oven is shut down, the slides are no longer transported to the oven after coverslipping; instead, they are deposited directly into the unload drawer.• Processing is shortened by about 5 minutes.WarningIf you shut down the oven, it is shut down immediately after pressing the Save button. Racks used earlier, and thus still in the process, go through the oven step in a slowly cooling oven. This can result in less effective drying and requires greater care when removing the slides from the rack.Switching on the oven1. To switch on the oven, press the Oven On (→ Fig. 34-1) button.2. If the oven is enabled, this is indicated by the button (→ Fig. 34-1) being colored red/white.3. Apply the activation by pressing the Save (→ Fig. 34-5) button and note the subsequent information message (→ Fig. 36) and confirm it with OK.Fig. 36 WarningAfter switching the oven back on, keep in mind that the oven takes up to 4 minutes to heat up. This can result in less effective drying for the initial racks and requires greater care when removing the slides from the rack.Excluding the oven step LThe oven step at the end of processing can be disabled if necessary. In this case, the oven remains switched on and can be made available immediately again if necessary by reactivating the oven step. The heating phase is omitted.1. To exclude the oven step, press the Exclude Oven Step (→ Fig. 34-3) button.
59HistoCore SPECTRA CVOperation 52. If the oven step is excluded, this is indicated by the button (→ Fig. 34-3) being colored red with an X.3. Apply the configuration by pressing the Save (→ Fig. 34-5) button and note the subsequent information message (→ Fig. 37) and confirm it with Ok.Fig. 37 WarningExcluding the oven step leads to less effective drying and requires greater care when removing slides from the rack.Including the oven step in the processing1. To enable the oven step at the end of processing, press the Exclude Oven Step (→ Fig. 34-3) button.2. If the oven step is enabled, no check mark in the (→ Fig. 34-3) button is displayed.3. Apply the configuration by pressing the Save (→ Fig. 34-5) button and note the subsequent information message (→ Fig. 38) and confirm it with Ok.Fig. 38
60 Version 1.2, Revision A Operation5Note • The function is valid only for racks inserted into the instrument after the Save button was pressed.• The overall processing is extended by the duration of the oven step (approx. 5 min.).The respective status of the oven is displayed in the status bar (→ Fig. 21):The oven is in the heating phaseThe oven is enabled and ready to operateThe oven is disabled5.8.6 Volume calibrationRequired access level: SupervisorThe application quantity of the mounting medium on the slide can be adapted to changes in ambient conditions by the Supervisor using the Volume Calibration menu.The properties of the mounting medium that is used can be changed depending on the ambient conditions. For example, the viscosity changes due to temperature differences. If the mounting medium is stored at a cool temperature and deployed in a cool state in the instrument for use, the viscosity is "tougher" (i.e. more viscous) than with a mounting medium which has already adapted to the ambient temperature. The viscosity has a direct influence on the application quantity of the mounting medium on the slide.If the application quantity differs during coverslipping so significantly that the user fears a loss of quality, the application quantity can be adjusted separately for both coverslip lines by means of the Volume Calibration menu. The application quantity configured in the parameter sets can remain unchanged. Note To adjust the application quantity, the password-protected Supervisor Mode is necessary. Furthermore, the adjustment is only possible if no processing is running and there are no longer any racks in the instrument.Adjusting the application quantity by the Supervisor1. Call up the Settings menu (→ Fig. 30-1) and press Volume calibration. 2. You can adjust the application quantity by turning the corresponding roller (→ Fig. 39-2) for coverslip line L1 or L2 (→ Fig. 39-1).
61HistoCore SPECTRA CVOperation 5Note Negative values (-1 through -5) reduce the application quantity for the selected coverslip line incrementally, while positive values (1 through 5) increase the application quantity incrementally.3. To apply the configured values, press the Save (→ Fig. 39-3) button for the respective coverslip line, L1 or L2.4. Note the subsequent information message and acknowledge it with Ok (→ Fig. 39-4).5. Exit the menu by pressing the Back (→ Fig. 39-5).6. To discard the settings, do not press the Save (→ Fig. 39-3) button, but instead press Back (→ Fig. 39-5) and exit the menu without saving.11225334Fig. 39 Volume calibration during installation is done by a Leica service technicianNote The pumps built into the coverslip lines have different flow rates due to the design. During the initial installation of the HistoCore SPECTRA CV, both pumps are matched by means of this menu.
62 Version 1.2, Revision A Operation55.8.7 Data managementRequired access level: Standard user, supervisor• Standard users can execute the User Export (→ Fig. 40-1) and Service Export (→ Fig. 40-2) functions.• The Import (→ Fig. 40-3) and SW Update (→ Fig. 40-4) functions can only be used by the Supervisor.The event logs (log files) can be exported in this menu. A USB memory stick connected to the USB port on the front side of the instrument (→ Fig. 2-7) is required for all exports and imports.12345Fig. 40 Note • The User Export, Service Export, Import and SW Update functions can only be carried out if the instrument is in sleep mode and there are no longer any racks in the instrument. Furthermore, the load and unload drawers must be closed.• The USB memory stick used must be formatted as FAT32.User export (→ Fig. 40-1)The User Export function is used to save information on a connected USB memory stick (→ Fig. 2-7):
63HistoCore SPECTRA CVOperation 5• A zip file with the event logs of the last 30 working days and CMS information in CSV format.• An encrypted lpkg file that contains all user-defined parameter sets and the consumables list.Note The encrypted lpkg file cannot be opened and viewed by the user.1. After pressing the User Export button, the data is exported to the connected USB memory stick.2. The information message User data is being exported... is displayed for the duration of the data export. The information message Export successful indicates to the user that the data transfer is finished and the USB memory stick can be removed safely. Press the OK button to close the message.Note If the message Export failed is displayed, an error occurred (e.g. the USB memory stick was removed too soon). In this case, the export process must be carried out again.Service export (→ Fig. 40-2)The Service Export function is used to save a lpkg file on a connected USB memory stick (→ Fig. 2-7). The encrypted lpkg file contains a predefined number of event logs as well as the following:• CMS information• User-defined parameter sets• Consumables data• Additional service-relevant dataNote The data is stored in encrypted form and can only be decrypted by a Leica service technician.• After pressing the Service export button, a selection menu appears in which the user can select the desired number of data records to be exported (5, 10, 15 or 30 days).• Press Ok to confirm the selection.• The information message Service data is being exported... is displayed for the duration of the data export.
64 Version 1.2, Revision A Operation5• The information message Export successful indicates to the user that the data transfer is finished and the USB memory stick can be removed safely.• If the message Export failed is displayed, an error occurred (e.g. the USB memory stick was removed too soon). In this case, the export process must be carried out again.Import (→ Fig. 40-3)Note The password-protected Supervisor Mode is required for importing. LThe function enables the import of language packages from a connected USB memory stick.1. To do this, insert the USB memory stick into one of the USB ports on the front side of the instrument.2. Then, select the Import function. The data is scanned.SW update (→ Fig. 40-4)Software updates can be run by a Supervisor or by a Leica-authorized service technician.Note Lab-specific settings are not deleted when updating the software for the HistoCore SPECTRA CV.5.8.8 Event viewRequired access level: Standard user, supervisorA separate log file is created for each day that the instrument was switched on. This file can be called up by selecting the DailyRunLog file in the Event view (→ Fig. 41).In the event viewer, an event log (→ Fig. 41) can be selected from a list of available logs (→ Fig. 41-1) and called up by pressing the Open (→ Fig. 41-2) button.
65HistoCore SPECTRA CVOperation 512Fig. 41 • All event entries begin with the Timestamp (→ Fig. 42-1), a date and time when the entry was created.• The title bars of the event viewer also indicate the serial number (→ Fig. 42-2) and the currently installed software version (→ Fig. 42-3) of HistoCore SPECTRA CV.• You can scroll up and down in the list and the log file using the arrow buttons (→ Fig. 42-4).• Pressing the left button allows you to scroll through the event viewer page by page.
66 Version 1.2, Revision A Operation5• Pressing the right button takes you to the beginning or the end of the event viewer.12344Fig. 42 5.9 Parameter settingsNote • The parameter set menu (→ Fig. 43) lets the user create new parameter sets, modify existing ones or assign a rack handle color to a parameter set. Creating or modifying a parameter set requires having the Supervisor user status and is only possible if there is no rack in the instrument and the instrument is idle.• The User status only allows one parameter set to be displayed.
67HistoCore SPECTRA CVOperation 51278 93 4 5 6Fig. 43 1Create a new parameter set 6Assigned rack handle color(s)2Parameter set number 7Editing a parameter set3Mounting medium used 8Deleting a parameter set4Coverglass length 9Assigning colors to a parameter set5Application volume5.9.1 Creating a new parameter set1. To create a new parameter set, press the New button (→ Fig. 43-1) in the parameter set menu.
68 Version 1.2, Revision A Operation52. In the following open menu, an incremental adjustment of the application volume (max. 5/min. -5) can take place (→ P. 72 – 5.9.5 Adjustment of the application volume). For this purpose set the desired volume by turning the roller (→ Fig. 44-1).3. Finally press the Save button (→ Fig. 44-2). The newly created parameter set is now saved and can be selected in the parameter set menu (→ Fig. 43). LTo discard the parameter set, press the Cancel (→ Fig. 44-3) button.123Fig. 44 5.9.2 Assigning a parameter set to a rack handle colorNote One or more rack handle color(s) can be assigned to each parameter set. For this purpose the Supervisor user status is required.1. In the parameter set menu (→ Fig. 45), tap on the corresponding entry to select the parameter set to which a rack handle color is to be assigned (→ Fig. 45-1).2. Pressing the Color (→ Fig. 45-2) button displays a selection field (→ Fig. 46), that allows the assignment of the rack handle color to the selected parameter set.Note The following must be taken into account when creating a parameter set when the workstation is working:• If slides in HistoCore SPECTRA ST are processed with a program, which was allocated a specific color (for example green), the user has to pay attention that the same color is assigned to the suitable parameter set in HistoCore SPECTRA CV which was created and is bootable.
69HistoCore SPECTRA CVOperation 5123Fig. 45 Note • All available colors are displayed in Figure (→ Fig. 46). If an abbreviation is entered in the color field, this color has already been assigned to a parameter set.• If an already assigned color is selected, a dialog box appears with a confirmation prompt indicating that the existing assignment will be undone. This can be confirmed with OK or canceled with Cancel.3. In the overview (→ Fig. 46-1), select a color that has not been assigned previously.
70 Version 1.2, Revision A Operation54. Save (→ Fig. 46-2) is used to assign the color and close the dialog. The selected color is now displayed in the parameter set list. LCancel (→ Fig. 46-3) is used to close the dialog without applying the changes.231Fig. 46 Note The color white is a wildcard color. When inserting a rack with a white handle, a selection window opens that has to be used to assign a unique parameter set to the white handle. Assigning the color white to a parameter set removes all colors previously assigned to that parameter set.5.9.3 Properties of the mounting mediumNote Leica provides validated mounting media for the HistoCore SPECTRA CV. This offers the user the following advantages:• Easy handling and non-contact filling and/or changing of the mounting medium• Low defect rate• Closed system• Fully automated importing of relevant data (designation, LOT numbers, number of possible coverslipping operations remaining) into CMS, • Graphical fill level display and monitoring.Designation Viscosity Drying time Based on...X1* Low Approx. 24 hours Xylene, Toluene*Ordering information: (→ P. 160 – Consumables)
71HistoCore SPECTRA CVOperation 5Note • Please observe the procedure when filling the mounting medium for the first time (→ P. 33 – 4.6 Refilling consumables).• Changing the mounting medium is described in (→ P. 79 – 6.3.1 Changing the mounting medium bottle).5.9.4 Properties of the coverglassLeica offers coverglass for the HistoCore SPECTRA CV, validated and manufactured in accordance with the ISO Standard 8255-1, 2011 (→ P. 20 – 3.2 Technical data). The Consumables Management System (CMS) automatically detects refilling and shows the current level of the coverglasses at all times.Only Leica coverglass* can be used in the HistoCore SPECTRA CV:Length in mm Width in mm Thickness50 24 #1*Ordering information: (→ P. 160 – Consumables)12345Fig. 47 1Coverglass size: 50 mm2Slides3Edge position of the coverglass on the slide (approx. 5 mm spacing)4Position on the slide, coverglass size of 50 mm5Label field of the slide
72 Version 1.2, Revision A Operation55.9.5 Adjustment of the application volumeThe HistoCore SPECTRA CV has a volume setting for the application volume of the mounting medium that is set by default and validated by Leica. The default values are defined as 0 (=default) in the setting for the parameter set (→ Fig. 48-1). These settings can be used immediately.Note Leica recommends checking the 0 value under the given conditions and requirements (e.g. specimen size, specimen type and specimen thickness, temperature and humidity) in the lab and adjusting it where appropriate.1. To adjust the application volume, select the corresponding parameter set in the Parameter sets menu and press the Edit button (→ Fig. 43-7).2. In the following menu (→ Fig. 48) the application volume can be lowered or increased by turning the roller (→ Fig. 48-1).3. The menu also offers the option of changing an assigned color (→ Fig. 48-2) or to assign a new color (→ P. 68 – 5.9.2 Assigning a parameter set to a rack handle color).4. Apply the settings you have entered by pressing the Save button (→ Fig. 48-3) or press Cancel (→ Fig. 48-4) to return to the Parameter sets menu without saving.1342Fig. 48 Set value SignificanceDefault value 0 Apply the Leica-validated quantity of mounting medium to the slide.Positive values 1 to 5 More mounting medium is applied incrementally to the slide. Negative values -1 to -5 Less mounting medium is applied incrementally to the slide.
73HistoCore SPECTRA CVOperation 5WarningChanges to the mounting medium application quantity should only be made with extreme caution to prevent negative effects on the coverslipping result.5.10 Reagent vessels in the load drawerWarning• It is absolutely necessary to ensure that the reagent filled in the reagent vessel is compatible with the mounting medium!• The user must monitor the levels of the reagent vessels to ensure they are sufficient.• If the instrument is to be in a non-running state for a moderate period of time or if the HistoCore SPECTRA CV is not to be used for a long period of time (longer than 5 days), cover or drain the reagent vessels in the load drawer in order to prevent evaporation of the filled reagent and to prevent the formation of vapors.• If the HistoCore SPECTRA CV is used in conjunction with the HistoCore SPECTRA ST as a workstation, the user must ensure that the last stations in the staining program of the HistoCore SPECTRA ST are filled with the same reagent as the load stations of the HistoCore SPECTRA CV.• Racks newly inserted into the load drawer are detected after closing the load drawer and indicated using the colors (→ Fig. 49-1).
74 Version 1.2, Revision A Operation51Fig. 49 • At the same time, the instrument checks whether the parameter set assigned to the rack handle color matches the mounting medium and coverslip size present in the coverslip line.• If this is not the case, a message prompts the user to take the rack back out of the load drawer and to place it in the other reagent vessel in the load drawer.• The instrument detects if a rack with a rack handle that has an unassigned color is inserted in either of the two reagent vessels. The user is prompted to take the rack back out and to create a corresponding parameter set (→ P. 67 – 5.9.1 Creating a new parameter set) or to assign the color to an appropriate existing parameter set (→ P. 68 – 5.9.2 Assigning a parameter set to a rack handle color).5.11 Module status• The menu Module Status (→ Fig. 50) provides the user in HistoCore SPECTRA CV an overview of the used consumables (mounting media (→ Fig. 50-1) and coverglass (→ Fig. 50-3)) and their current fill levels (→ Fig. 50-5) (→ Fig. 50-6) and remaining amounts (→ Fig. 50-2) (→ Fig. 50-4) for both coverslip lines.• The user has access to various cleaning options for the hose system through the Prime/Clean submenu (→ Fig. 50-7).
75HistoCore SPECTRA CVOperation 51623475Fig. 50 1Mounting medium designation 5Remaining amount of mounting medium in %2Remaining number of slides 6Remaining amount of coverglasses in %3Coverglass length 7Prime/Clean submenu4Remaining number of coverglassNote For more information about the Prime/Clean submenu and using the individual priming and cleaning options: (→ P. 122 – 7.3 Preparing the hose system for priming and cleaning).

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